Parani, M. and Rajesh, K. and Lakshmi, M. and Parducci, L. and Szmidt, A.E. and Parida, A. (2001) Species identification in seven small millet species using polymerase chain reaction – restriction fragment length polymorphism of trnS-psbC gene region. Genome, 44 (3). pp. 495-499.
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Abstract
The chloroplast trnS-psbC gene regions from total genomic DNA of 119 accessions from seven small millet species were amplified by polymerase chain reaction (PCR) and digested with eight restriction enzymes individually as well as in combinations of two enzymes to generate restriction fragment length polymorphism (PCR–RFLP). PCR–RFLP with individual enzymes revealed polymorphism between only some species. However, all the species could be distinguished by using a combination of two enzymes, specifically HaeIII and MspI. PCR–RFLP of 11 to 20 accessions with the same enzyme combination showed no intraspecific variation, which established that the differential banding patterns were species specific. In contrast, the same enzyme combination was not useful for differentiating different species of the genera Cajanus, Rhyncosia, Abies, Rhizophora, Ceriops, and Bruguiera, and it also revealed intraspecies variation in three species of Abies. The present study indicated that digestion of trnS-psbC with two four-base recognizing enzymes reveals more variation than with either enzyme alone and that it may be a method of choice for species identification in some genera.
| Item Type: | Article |
|---|---|
| Author Affiliation: | M.S. Swaminathan Research Foundation, III Cross Street, Institutional Area, Taramani, Chennai 600 113, India |
| Subjects: | Crop Improvement Crop Improvement > Genetics/Genomics |
| Divisions: | Millet |
| Depositing User: | Sandhya Gir |
| Date Deposited: | 22 Dec 2010 16:59 |
| Last Modified: | 29 Dec 2010 19:35 |
| Official URL: | http://article.pubs.nrc-cnrc.gc.ca/RPAS/rpv?hm=HIn... |
| URI: | http://eprints.icrisat.ac.in/id/eprint/1125 |
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