Wagih, E.E. and Melouk, H.A. and Sherwood , J.L. (1994) Isolation and Partial Characterization of Peanut Top Paralysis Virus (PTPV), a Destructive Virus Causing a New Disease in Peanut (Arachis hypogaea L.). Journal of Phytopathology, 141 (3). pp. 302-313.
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Abstract
A virus causing top paralysis in groundnut was discovered in the wild germplasm collection growing in the USDA-ARS greenhouses, Stillwater, Oklahoma, USA. Symptoms on the wild plant were restricted to a few leaves and were observed as green patches in a light green to yellow background with some leaflets having lost most of the basal part of the laminae leaving the top portion rolling upwards forming a cone. The virus was mechanically transmitted to cultivated groundnut where it caused more severe and destructive symptoms including stunting, severe malformation of leaves and partial or complete disappearance of leaflet laminae. This virus differed in symptomatology, host range and/or serological reactivity from all groundnut viruses reported in the literature, particularly those causing leaf malformation and stunting. The virus included necrotic local lesions on Phaseolus vulgaris cv. Topcrop and chlorotic lesions with necrotic centres bordered with very bright intense red colour on Chenopodium amaranticolor. In both passive indirect ELISA and Ouchterlony double immunodiffusion tests, the virus did not react with antisera against brome mosaic bromovirus, bean yellow mosaic potyvirus, peanut stripe potyvirus, potato Y potyvirus, tobacco mosaic tobamovirus, watermelon mosaic 1 virus [papaya ringspot potyvirus], watermelon mosaic 2 potyvirus, wheat soil-borne mosaic virus [soil-borne wheat mosaic furovirus], wheat streak mosaic rymovirus and zucchini yellow mosaic potyvirus. However, in reciprocal cross reactions the virus seemed to share a common antigenic determinant with a peanut mottle potyvirus isolate from Oklahoma (PMV-OK). The virus had flexuous filamentous particles of 750-850 nm in length, falling within the range reported for the potyvirus group. The virus was successfully purified and the MW of its protein subunit was found to be 30 000 d. A polyclonal antiserum was raised in rabbits against the virus and used for reciprocal serological tests
| Item Type: | Article |
|---|---|
| Additional Information: | SNNigam Collection |
| Author Affiliation: | Department of Plant Pathology, College of Agriculture, University of Alexandria, Alexandria, Egypt |
| Subjects: | Plant Protection |
| Divisions: | Groundnut |
| Depositing User: | Mr B Krishnamurthy |
| Date Deposited: | 25 Jul 2013 07:59 |
| Last Modified: | 25 Jul 2013 07:59 |
| Official URL: | http://dx.doi.org/10.1111/j.1439-0434.1994.tb01474... |
| URI: | http://eprints.icrisat.ac.in/id/eprint/11225 |
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