Dietzgen, R.G. and Teycheney, P.Y. and Birch, R.G. and Livingstone, M. (1993) Molecular Cloning of Peanut Stripe Potyvirus Coat Protein Gene and Progress in Marker Gene Transfer and Peanut Regeneration. ACIAR Food Legume Newsletter, 18. pp. 11-12.
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Abstract
A library of recombinant cDNA bacterial clones was established and plasmids carrying sequences near the 3′ terminus of the viral RNA which include the coat protein gene were identified. The complete nucleotide sequence of the PStV CP gene and 3′ untranslated region of the Indonesian blotch isolate was determined in both directions from 3′ terminal cDNA clones and subclones. The PStV CP gene is 861 nucleotides long and can potentially encode a protein of 287 amino acids of Mr 31 500 Da. Explants and tissue culture conditions for providing suitable target material for gene transfer and plant regeneration were identified and conditions established for obtaining plants from de-embryonated cotyledons and embryonic leaflets of 2 groundnut cultivars
| Item Type: | Article |
|---|---|
| Additional Information: | SNNigam Collection |
| Author Affiliation: | Queensland Department of Primary Industries, Agricultural Biotechnology Centre, University of Queensland, St Lucia, Qld 4072, Australia |
| Subjects: | Plant Protection Crop Improvement |
| Divisions: | Groundnut |
| Depositing User: | Mr B Krishnamurthy |
| Date Deposited: | 25 Jul 2013 07:45 |
| Last Modified: | 25 Jul 2013 07:45 |
| URI: | http://eprints.icrisat.ac.in/id/eprint/11215 |
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