Tsvetova, M.I. and Elkonin, L.A. (2013) Cytological Investigation of Pollen Development in Sorghum Line with Male Sterility Induced by Sodium Ascorbate in Tissue Culture. American Journal of Plant Sciences, 4. pp. 11-18.
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Abstract
Investigation of male sterility mutations is an effective approach for identification of genes involved in anther and pollen development. The comparison of “cytological phenotypes” of newly induced mutants with phenotypes determined by already known genes favors elucidation of genetic control of diverse microsporo- and gametogenesis stages. In this paper, we describe pollen development in the grain sorghum line Zh10-asc1 with mutation of male sterility. This line was obtained from callus culture treated by sodium ascorbate. A wide spectrum of abnormalities in microsporogenesis have been found, such as cytomixis, chromosomal laggards, chromosome disjunction, adhesion of chromosomes, disturbed cytokinesis, and others. In tapetum, the cells with one nucleus, with unequal nuclei, and with micronuclei have been observed. During pollen grain (PG) maturation abnormalities in starch accumulation and delay of development often took place. In mature anthers, a variety of pollen grain types have been revealed: fertile, of irregular shape, incompletely filled with starch, PGs delayed at the uni-nucleate or bi-nucleate gametophyte stages, with partially or fully degenerated contents, and with abnormal coloration. Variation in spectrum and the frequency of disturbances between the flowers of one and the same plant have been revealed. The reasons for significant genetic and epigenetic instability are discussed.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Male Sterility; Microsporogenesis; Sodium Ascorbate; In Vitro Mutagenesis; Genetic Instability; Sorghum bicolor (L.) Moench |
| Author Affiliation: | Department of Biotechnology, Agricultural Research Institute for South-East Region, Saratov, Russia |
| Subjects: | Statistics and Experimentation Crop Improvement |
| Divisions: | Sorghum |
| Depositing User: | Mr. SanatKumar Behera |
| Date Deposited: | 20 Jul 2013 09:11 |
| Last Modified: | 20 Jul 2013 09:11 |
| Official URL: | http://dx.doi.org/10.4236/ajps.2013.47A1002 |
| URI: | http://eprints.icrisat.ac.in/id/eprint/11180 |
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