Genetic variation detectable with molecular markers among unadapted germ-plasm resources of cultivated peanut and related wild species

Halward, T.M. and Stalker, H.T. and LaRue, E.A. and Kochert , G. (1991) Genetic variation detectable with molecular markers among unadapted germ-plasm resources of cultivated peanut and related wild species. Genome, 34 (6). pp. 1013-1020.

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Abstract

Genetic variation was studied in unadapted groundnut germplasm lines from the various South American centres of origin, Africa and China, where considerable morphological and physiological variability has been reported to exist. Wild species of section Arachis were included in the evaluations since they show a high degree of variation when assayed by RFLPs. Three methods were used to assay for RFLP variation: (1) conventional RFLP analysis using random genomic clones from groundnut and cDNA clones from groundnut and lucerne (Medicago sativa); (2) polymerase chain reaction (PCR) amplification of random primer sequences; and (3) four-cutter analysis of PCR-amplified fragments. In all cases a very low level of variability was found in cultivated groundnut, while abundant variability was present among wild diploid species

Item Type: Article
Additional Information: SNNigam collection
Author Affiliation: Department of Botany, University of Georgia, Athens, GA 30602, USA.
Subjects: Crop Improvement
Divisions: Groundnut
Depositing User: Mr T L Gautham
Date Deposited: 13 Jul 2013 14:17
Last Modified: 13 Jul 2013 14:17
Official URL: http://dx.doi.org/10.1139/g91-156
URI: http://eprints.icrisat.ac.in/id/eprint/11037

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