Chemical screening for Aspergillus flavus resistance in peanuts

Amaya-F, J. and Young, C.T. and Norden, A.J. and Mixon, A.C. (1980) Chemical screening for Aspergillus flavus resistance in peanuts. Oleagineux, 35 (5). pp. 255-257.

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Abstract

A chemical method based on the content of soluble amino compounds in the seed coat of individual groundnut seeds for screening them for resistance to A. flavus attack, is described. Aqueous extracts of c. 10 mg of the seed coat were hydrolysed in 6 N HC1 at 145 deg C for 2 h and the total amino acids determined by the reaction with o-phthalaldehyde. Using the method with 18 groundnut cv. and lines, the results agreed closely with microbiological tests in 16 of them.ADDITIONAL ABSTRACT:C. 10 mg of the testa of groundnuts were extracted with water, the extracts hydrolysed in 6N HCl at 145 deg C for 2 h and the total amino acids estimated by the o-phthalaldehyde reaction. Results (low levels indicating resistance) agreed closely with microbiological data for 16 and 18 lines tested. The method can be used to screen for resistance and control of aflatoxin production on an individual seed basis.ADDITIONAL ABSTRACT:In 18 lines or varieties of groundnut of known resistance or susceptibility to A. flavus grown in two localities in the USA or in Puerto Rico, the total content of soluble amino compounds in the testa was correlated with susceptibility, except in two lines, UF75515 and Tifspan, which were highly susceptible and which had a low content. In a further study of 12 lines or varieties, including UF75515 and Tifspan, susceptibility was associated with a low content of acid-hydrolysable arabinose in the testa.

Item Type: Article
Additional Information: SNNigams colletion
Author Affiliation: Dep. of Food Sci., Georgia Univ., Experiment, GA 30212, USA.
Subjects: Crop Improvement
Plant Physiology and Biochemistry > Biochemistry
Divisions: Groundnut
Depositing User: Mr T L Gautham
Date Deposited: 08 Jul 2013 13:49
Last Modified: 05 Aug 2013 05:08
URI: http://eprints.icrisat.ac.in/id/eprint/10975

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