Talaei, S. and Asadi , A. and Amani , M. (2013) Competitive inhibition of copper amine oxidases by vitamin B hydrochloride in chickpea. Molecular Biology Research Communications, 2 (1-2). pp. 29-38.
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Abstract
Copper amine oxidases (CAOs) catalyse the oxidative de-amination of biogenic amines which are ubiquitous compounds essential for cell growth and proliferation. The enzymes are homodimers containing both topaquinone and a Cu(II) ions as cofactors at the active site of each subunit. After extraction and purification of chickpea (cicer arietinum) amine oxidase by chromatoghraphy, Km and Vmax of the enzyme were determined to be 3.3 mM and 0.95 mmol/min/mg, respectively, using a Lineweaver-Burk plot. In this study, the interaction of chickpea diamino oxidase with vitamin B hydrochloride was studied. Vitamin B hydrochloride (Thiamin) by Ki=8 mM acted as competitive inhibitor of the enzyme.
| Item Type: | Article |
|---|---|
| Author Affiliation: | University of Mohaghegh Ardabili, Ardabil, Iran |
| Subjects: | Postharvest Management > Food Technology Plant Physiology and Biochemistry > Biochemistry |
| Divisions: | Chickpea |
| Depositing User: | Mr. SanatKumar Behera |
| Date Deposited: | 08 Jul 2013 03:34 |
| Last Modified: | 08 Jul 2013 03:34 |
| Official URL: | http://mbrc.shirazu.ac.ir/?_action=articleInfo&art... |
| URI: | http://eprints.icrisat.ac.in/id/eprint/10942 |
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