Surekha, Ch. and Arundhati, A. and Rao, G.S. (2007) Differential response of Cajanus cajan varieties to transformation with different strains of agrobacterium. Journal of Biological Sciences, 7 (1). pp. 176-181.
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Abstract
Stable transformation followed by regeneration and subsequent expression of genes is important in the production of transgenic plants. Plumule and nodal segments of embryo, cotyledons and nodes from 10-12 day old seedlings of pigeonpea genotypes were cocultivated with different strains of Agrobacterium tumefaciens containing Ti plasmid and gus reporter gene. Transformation frequencies measured as number of explants showing gus expression was studied. It was observed that the transformation frequency is explant, genotype and Agrobacterium strain dependent. The transformation frequency was higher in ICPL87 and nodal explant in all genotypes studied. Agrobacterium strain LBA4404 was more compatible in giving higher frequency of transformants with all explants of all genotypes.
| Item Type: | Article |
|---|---|
| Author Affiliation: | Department of Biochemistry, College of Science, GITAM, Visakhapatnam |
| Subjects: | Plant Production > Varieties Crop Improvement > Genetics/Genomics |
| Divisions: | Pigeonpea |
| Depositing User: | Mr T L Gautham |
| Date Deposited: | 20 Dec 2010 17:39 |
| Last Modified: | 20 Dec 2010 17:39 |
| Official URL: | http://scialert.net/qredirect.php?doi=jbs.2007.176... |
| URI: | http://eprints.icrisat.ac.in/id/eprint/1074 |
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