Electrophoretic analysis of malting degradability of major sorghum reserve proteins

Mokhawa, G and Kerapeletswe-Kruger, C.K. and Ezeogu, L.I (2013) Electrophoretic analysis of malting degradability of major sorghum reserve proteins. Journal of Cereal Science.

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Abstract

Proteolysis is vital to the generation of amino acids and short peptides during malting. The qualitative and quantitative effects of malting on proteolytic digestion were investigated for 11 Botswana sorghum cultivars. Protein hydrolysis was influenced by sorghum grain cultivar. All protein fractions were degraded, although the extents of their digestion appeared cultivar-dependent. The most significant changes in total and free kafirins occurred among the HMW aggregates while the 45 kDa dimer appeared the most recalcitrant. Free kafirin monomers were digested in variety-defined manner; α-kafirin faded the most (ca. 82%) in Phofu, but appeared least digested (≤10%) in Lekgeberwa; β-kafirin faded the most in Phofu and Segaolane (90-94%) but only 46% in Lekgeberwa. Overall, α-kafirin appeared the least digested of the free monomers. The proportion of free kafirin accounted for by the 45 kDa and monomeric kafirins increased in all malts (except Lars Vyt and Mafia), probably due to protein depolymerisation. Grain variety significantly influenced (p <0.001) malt FAN levels. Mean FAN values were highest in Segaolane (225 mg/100 g) followed by Sefofu, Town and BSH-1, but lowest in Mafia (96.9 mg/100 g). Results indicate wide variability in the proteolytic malting digestibility of the eleven sorghum cultivars.

Item Type: Article
Uncontrolled Keywords: Sorghum malting; Proteolysis; Kafirin; Free α-amino nitrogen
Author Affiliation: National Food Technology Research Centre, 1840 Mpuutsane Industrial Area, P/Bag 008, Kanye, Republic of Botswana
Subjects: Postharvest Management > Food Technology
Divisions: Sorghum
Depositing User: Mr Balakrishna Garadasu
Date Deposited: 25 Jun 2013 10:42
Last Modified: 25 Jun 2013 10:42
Official URL: http://dx.doi.org/10.1016/j.jcs.2013.05.008
URI: http://eprints.icrisat.ac.in/id/eprint/10736

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