Gao, J. and Xia, B. and Luo, F. and et al, . (2013) Marker-assisted breeding for rf1, a nuclear gene controlling A1 CMS in sorghum (Sorghum bicolor L. Moench). Euphytica, 193 (3). pp. 383-390.
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Abstract
A study on marker-assisted selection (MAS) for the rf1 gene, which controls pollen sterility in the sorghum A1 cytoplasm, was conducted on the offspring population of two crosses between a maintainer line, BTx-622, and two sweet sorghum lines, BJ-299 and Lunen-2, to test the effectiveness of the MAS method and develop maintainer lines with sweet and juicy stalks and corresponding cytoplasmic-nuclear male sterility (CMS) lines. The simple sequence repeat marker Xtxp18 exhibited a high accuracy (95.098 %) for selecting recessive homozygotes for the rf1 gene. The segregation ratio matched the expected ratio calculated according to the reported genetic distance in the F2 population of the two crosses used. Finally, four excellent maintainer lines/CMS line pairs (F5/BC3) with high stalk juice and stalk juice sugar contents were developed. The MAS method based on Xtxp18 for the sorghum rf1 gene could be used for hybrid breeding programs at a low cost in the future.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Sorghum, Cytoplasmic-nuclear male sterility, rf1 gene Marker-assisted selection, Stalk juice sugar content Stalk juice content |
| Author Affiliation: | Institute of Animal Science, Chinese Academy of Agricultural Science, Beijing 100193, People’s Republic of China ; e-mail: lxl@caas.net.cn |
| Subjects: | Crop Improvement |
| Divisions: | Sorghum |
| Depositing User: | Mr Siva Shankar |
| Date Deposited: | 30 May 2013 04:31 |
| Last Modified: | 16 Sep 2013 11:07 |
| Official URL: | http://dx.doi.org/10.1007/s10681-013-0939-6 |
| URI: | http://eprints.icrisat.ac.in/id/eprint/10662 |
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