Characterization of root-nodule bacteria isolated from Vicia faba and selection of plant growth promoting isolates

Saïdi, S. and Chebil, S. and Gtari, M. and Mhamdi, R. (2013) Characterization of root-nodule bacteria isolated from Vicia faba and selection of plant growth promoting isolates. World Journal of Microbiology and Biotechnology, 29 (6). pp. 1099-1106.

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Abstract

A collection of 104 isolates from root-nodules of Vicia faba was submitted to 16S rRNA PCR–RFLP typing. A representative sample was further submitted to sequence analysis of 16S rRNA. Isolates were assigned to 12 genera. All the nodulating isolates (45 %) were closely related to Rhizobium leguminosarum USDA2370T (99.34 %). The remaining isolates, including potential human pathogens, failed to nodulate their original host. They were checked for presence of symbiotic genes, P-solubilization, phytohormone and siderophore production, and then tested for their growth promoting abilities. Results indicated that 9 strains could induce significant increase (41–71 %) in shoot dry yield of faba bean. A Pseudomonas strain was further assessed in on-farm trial in combination with a selected rhizobial strain. This work indicated that nodule-associated bacteria could be a valuable pool for selection of effective plant growth promoting isolates. Nevertheless, the possible involvement of nodules in increasing risks related to pathogenic bacteria should not be neglected and needs to be investigated further

Item Type:	Article
Author Affiliation:	Laboratory of Legumes, Centre of Biotechnology of Borj-Cédria, BP 901, 2050, Hammam-Lif, Tunisia
Subjects:	Soil Science and Microbiology > Microbiology Crop Improvement > Biotechnology
Divisions:	Other Crops
Depositing User:	Ms K Syamalamba
Date Deposited:	27 May 2013 08:59
Last Modified:	27 May 2013 08:59
Official URL:	http://link.springer.com/article/10.1007/s11274-01...
URI:	http://eprints.icrisat.ac.in/id/eprint/10639

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