Palapala, V.A. and Aimi, T. and Inatomi, S. and Morinaga, T. (2002) ITS-PCR-RFLP Method for Distinguishing Commercial Cultivars of Edible Mushroom, Flammulina velutipes. Journal of Food Science, 67 (7). pp. 2486-2490.
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Abstract
Nucleotide sequence and polymerase chain reaction (PCR) - restriction fragment length polymorphism (RFLP) analysis of the ribosomal RNA gene (rDNA) regions containing the internal transcribed spacers (ITSs) and the 5.8S rRNA coding sequence was used to differentiate between 7 typical Flammulina strains. These nucleotide sequences revealed the presence of strain-specific deletions, insertions, and substitutions. Moreover, RFLP patterns produced using restriction endonucleases DraI, FokI, HaeII, MboII, and NlaIV, enabled identification of specific Flammulina strains. Thus, PCR-RFLP analysis of the ITS regions appears to be a useful tool for the identification of Flammulina strains.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Flammulina velutipes;PCR-RFLP;edible mushroom;rDNA;ITS |
| Author Affiliation: | Dept. of Bioresource Development, Hiroshima Prefectural Univ., Nanatsuka-cho, Shobara-shi, Hiroshima, 727-0023, Japan |
| Subjects: | Crop Improvement |
| Divisions: | Other Crops |
| Depositing User: | Mr Siva Shankar |
| Date Deposited: | 27 May 2013 08:26 |
| Last Modified: | 27 May 2013 08:26 |
| Official URL: | http://dx.doi.org/10.1111/j.1365-2621.2002.tb08763... |
| URI: | http://eprints.icrisat.ac.in/id/eprint/10638 |
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