Identification of Ochratoxigenic Aspergillus Section Nigri Isolated from Grapes by ITS-5.8S rDNA Sequencing Analysis and In Silico RFLP

Lasram, S. and Oueslati, S. and Jouira, H.B. and et al, . (2013) Identification of Ochratoxigenic Aspergillus Section Nigri Isolated from Grapes by ITS-5.8S rDNA Sequencing Analysis and In Silico RFLP. Journal of Phytopathology, 161 (4). pp. 280-283.

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Abstract

To characterize Aspergillus section Nigri strains involved in the ochratoxin A (OTA) contamination of Tunisian wine and table grapes, a total of 33 strains were analysed. A molecular characterization of the isolates was performed by the amplification of internal transcribed spacer (ITS1-5.8S rDNA-ITS2) region combined with amplicon sequencing. Analysis of similarity between the obtained sequences and those deposited in the GenBank database was performed. Twelve strains were confirmed to belong to the Aspergillus carbonarius species. Strains belonging to the Aspergillus niger aggregate group were classified by in silico RFLP assay into two patterns N and T, corresponding to A. niger and Aspergillus tubingensis. Among the 21 OTA producing isolates analysed, 13 showed the T-type pattern and 8 showed the N-type pattern. The presented method showed to be a reliable alternative to the classic RFLP method. Our findings unambiguously revealed that multiple aspergilli species isolated from wine and table grape in Tunisia are able to produce OTA.

Item Type: Article
Uncontrolled Keywords: A. carbonarius ; A. niger ; A. tubingensis ; in silico RFLP
Author Affiliation: Laboratory of Molecular Physiology of Plants – Borj-Cedria Biotechnology Center, Tunisia
Subjects: Plant Physiology and Biochemistry > Plant Physiology
Divisions: General
Depositing User: Mr Siva Shankar
Date Deposited: 27 May 2013 08:11
Last Modified: 27 May 2013 08:18
Official URL: http://dx.doi.org/10.1111/jph.12044
URI: http://eprints.icrisat.ac.in/id/eprint/10635

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