Shan, Q. and Wang, Y. and Chen, K. and et al, . (2013) Rapid and Efficient Gene Modification in Rice and Brachypodium Using TALENs. Molecular Plant. pp. 1-4.
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Abstract
In the past few years, the use of sequence-specific nucleases for efficient targeted mutagenesis has provided plant biologists with a powerful new approach for understanding gene function and developing new traits. These nucleases create DNA double-strand breaks at chromosomal targeted sites that are primarily repaired by the non-homologous end joining (NHEJ) or homologous recombination (HR) pathways. NHEJ is often imprecise and can introduce mutations at target sites resulting in the loss of gene function. In contrast, HR uses a homologous DNA template for repair and can be employed to create site-specific sequence modifications or targeted insertions (Moynahan and Jasin, 2010).
| Item Type: | Article |
|---|---|
| Author Affiliation: | State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China |
| Subjects: | Crop Improvement |
| Divisions: | Other Crops |
| Depositing User: | Mr Balakrishna Garadasu |
| Date Deposited: | 25 May 2013 14:02 |
| Last Modified: | 25 May 2013 14:04 |
| Official URL: | http://dx.doi.org/10.1093/mp/sss162 |
| URI: | http://eprints.icrisat.ac.in/id/eprint/10594 |
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