Nikus, J. and Danie, G. and Jonsson, L.M.V. (2001) Subcellular localization of β-glucosidase in rye, maize and wheat seedlings. Physiologia Plantarum, 111 (4). pp. 466-472.
![[img]](http://eprints.icrisat.ac.in/style/images/fileicons/application_pdf.png) |
PDF
- Published Version
Restricted to ICRISAT researchers only |
Abstract
The subcellular compartmentation of β-glucosidase was studied in rye, maize and wheat seedlings by immunocytochemical methods. For detection, we used a 10 nm gold-labeled secondary antibody, and results were observed using transmission electron microscopy. In all three species, β-glucosidase was found in plastids, cytoplasm and cell walls. In rye, gold particles were seen on cell walls and cytoplasm in epidermal cells of the root tip and shoot, in bundle sheath cells of the shoot and in all cells, except the vascular bundle cells of the coleoptile. Gold labeling was also observed in plastids of the bundle sheath cells of rye shoot tips and in cortical cells of root tips. In wheat, gold labeling was observed on cell walls and cytoplasm of epidermal cells in the shoot base and coleoptile, and on cell walls and plastids in epidermal cells of the root tip. In maize, gold labeling was mainly found in plastids or proplastids in vascular bundle cells and bundle sheath cells of the shoot, in bundle sheath cells of the coleoptile and in epidermal cells of the root. Some gold particles were also found in cell walls and cytoplasm of stomatal guard cells of the shoot base and vascular bundle cells of the shoot tip and in the cell walls of bundle sheath cells of the shoot tip and root tip epidermal cells. Results are discussed in relation to the role of β-glucosidase in hydroxamic acid release and overall defense mechanism of monocotyledons.
| Item Type: | Article |
|---|---|
| Author Affiliation: | Swedish University of Agricultural Sciences, Box 7080, SE-750 07 Uppsala, Sweden |
| Subjects: | Crop Improvement Plant Physiology and Biochemistry > Biochemistry |
| Divisions: | Maize |
| Depositing User: | Mr. SanatKumar Behera |
| Date Deposited: | 20 May 2013 06:16 |
| Last Modified: | 20 May 2013 06:16 |
| Official URL: | http://dx.doi.org/ 10.1034/j.1399-3054.2001.111040... |
| URI: | http://eprints.icrisat.ac.in/id/eprint/10570 |
Actions (login required)
 |
View Item |
