Segovia, L. and Piñero, D. and Palacios, R. and Martínez-Romero, E. (1991) Genetic structure of a soil population of nonsymbiotic Rhizobium leguminosarum. Applied and Environmental Microbiology, 57 (2). pp. 426-433.
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Abstract
The genetic structure of a population of nonsymbiotic Rhizobium leguminosarum strains was determined by the electrophoretic mobilities of eight metabolic enzymes. Nonsymbiotic strains were isolated from the rhizosphere of bean plants and characterized by growth on differential media and at different temperatures, intrinsic antibiotic resistance, the lack of homology to a nifH probe, and their inability to form nodules on bean roots. All the isolates clustered with R. leguminosarum bv. phaseoli reference strains and did not encompass any other Rhizobium taxa. Their rRNA operon restriction fragment length polymorphisms and the nucleotide sequence of a fragment of the 16S rRNA gene were also found to be identical to those of R. leguminosarum bv. phaseoli reference strains. When complemented with an R. leguminosarum bv. phaseoli symbiotic plasmid (p42d), the nonsymbiotic isolates were able to fix nitrogen in symbiosis with bean roots at levels similar to those of the parental strain. The symbiotic isolates were found at a relative frequency of 1 in 40 nonsymbiotic R. leguminosarum strains
| Item Type: | Article |
|---|---|
| Author Affiliation: | Departamento de Genética Molecular, Universidad Nacional Autónoma de México, Cuernavaca, Morelos |
| Subjects: | Soil Science and Microbiology Crop Improvement |
| Divisions: | General |
| Depositing User: | Mr B Krishnamurthy |
| Date Deposited: | 14 May 2013 11:23 |
| Last Modified: | 14 May 2013 11:23 |
| URI: | http://eprints.icrisat.ac.in/id/eprint/10536 |
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