Identification of polymorphic DNA markers in cultivated peanut (Arachis hypogaea L.)

He, G. and Prakash , C.S. (1997) Identification of polymorphic DNA markers in cultivated peanut (Arachis hypogaea L.). Euphytica , 97 (2). pp. 143-149.

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Abstract

The detection of DNA polymorphism in cultivated peanut (Arachis hypogaea L.) is reported here for the first time. The DNA amplification fingerprinting (DAF) and amplified fragment length polymorphism (AFLP) approaches were tested for their potential to detect genetic variation in peanut. The AFLP approach was more efficient as 43% of the primer combinations detected polymorphic DNA markers in contrast to 3% with the DAF approach. However, the number of polymorphic bands identified using primers selected in both approaches was comparable. In the DAF study, when 559 primers of varying types were screened, 17 (mostly 10-mer types) detected polymorphism producing an average of 3.7 polymorphic bands per primer with a total of 63 polymorphic markers. In the AFLP study, when 64 primer combinations (three selective nucleotides) corresponding to restriction enzymes Eco RI and Mse I were screened, 28 detected polymorphism. On an average, 6.7% of bands obtained from these 28 primer pairs were polymorphic resulting in a total of 111 AFLP markers. Our results demonstrate that both AFLP and DAF approaches can be employed to generate DNA markers in peanut and thus have potential in the marker-assisted genetic improvement and germplasm evaluation of this economically important crop

Item Type: Article
Additional Information: SNNigam Collection
Uncontrolled Keywords: amplified fragment length polymorphism (AFLP) markers, DNA amplification fingerprinting (DAF), groundnut, polymerase chain reaction (PCR), random amplified polymorphic DNA (RAPD) markers
Author Affiliation: Center for Plant Biotechnology Research, Tuskegee University, School of Agriculture and Home Economics, Milbank Hall, Tuskegee, AL 36088, U.S.A.
Subjects: Crop Improvement
Divisions: Groundnut
Depositing User: Mr B Krishnamurthy
Date Deposited: 18 Apr 2013 05:28
Last Modified: 18 Apr 2013 05:28
Official URL: http://dx.doi.org/10.1023/A:1002949813052
URI: http://eprints.icrisat.ac.in/id/eprint/10277

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