Tiwari, S. and Tuli , R. (2009) Multiple shoot regeneration in seed-derived immature leaflet explants of peanut (Arachis hypogaea L.). Scientia Horticulturae, 121 (2). pp. 223-227.
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Abstract
A protocol was developed for organogenesis from immature leaflet explants derived from mature seeds of peanut. Immature leaflets pre-incubated on MS medium supplemented with 13.32 µM BAP+4.95 µM NAA for 7 days, turned green and enlarged. The enlarged green leaflets produced multiple shoot buds after 1-2 cycles of sub-culture on MS medium supplemented with 13.32 µM BAP. Three cycles of shoot buds on the elongation medium (13.32 µM BAP) produced 6.17±0.47 elongated shoots per explant. The shoot bud formation was genotype independent. All elongated shoots rooted on the medium containing 4.95 µM NAA. The complete protocol gave efficient (>81%) direct organogenesis, leading to the development of plantlets within 4 months
| Item Type: | Article |
|---|---|
| Additional Information: | SNNigam Collection |
| Uncontrolled Keywords: | Organogenesis, Peanut, Phytohormone, Plant regeneration |
| Author Affiliation: | Plant Molecular Biology and Genetic Engineering Division, National Botanical Research Institute, Council of Scientific and Industrial Research, Rana Pratap Marg, Lucknow 226 001, U.P., India |
| Subjects: | Crop Improvement |
| Divisions: | Groundnut |
| Depositing User: | Mr B Krishnamurthy |
| Date Deposited: | 18 Apr 2013 05:20 |
| Last Modified: | 18 Apr 2013 05:20 |
| Official URL: | http://dx.doi.org/10.1016/j.scienta.2009.01.029 |
| URI: | http://eprints.icrisat.ac.in/id/eprint/10268 |
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