Tiwari, S. and Mishra, D.K. and Singh, Ankit and Singh, P.K. and Tuli, R. (2008) Expression of a synthetic cry1EC gene for resistance against Spodoptera litura in transgenic peanut (Arachis hypogaea L.). Plant Cell Reports, 27 (6). pp. 1017-1025.
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Abstract
The tobacco cutworm (Spodoptera litura) is a polyphagous foliage insect and a major pest on peanut (Arachis hypogaea L.). S. litura is susceptible to the chimeric δ-endotoxin Cry1EC reported earlier. De-embryonated cotyledon explants of peanut were transformed using Agrobacterium tumefaciens strain EHA101 harboring a synthetic cry1EC gene driven by the CaMV 35S promoter. Transgenic plants of peanut with a single copy insertion of cry1EC were selected in the T0 generation by Southern blot hybridization. Real-time PCR, Western blot and ELISA analysis indicated that expression of the cry1EC gene was higher in single copy T1 plants. Immunoassay showed expression of Cry1EC up to 0.13% of total soluble protein in T1 plants. Leaf feeding bioassay on highly expressing transgenic lines showed 100% killing of larvae at the 2nd instar stage of S. litura
| Item Type: | Article |
|---|---|
| Additional Information: | SNNigam Collection |
| Uncontrolled Keywords: | Agrobacterium tumefaciens, Arachis hypogaea, cry1EC, De-embryonated cotyledon, Real-time PCR, Spodoptera litura |
| Author Affiliation: | Plant Molecular Biology and Genetic Engineering Division, National Botanical Research Institute, Rana Pratap Marg, Lucknow, UP, 226 001, India |
| Subjects: | Plant Protection Crop Improvement |
| Divisions: | Groundnut |
| Depositing User: | Mr B Krishnamurthy |
| Date Deposited: | 18 Apr 2013 04:40 |
| Last Modified: | 18 Apr 2013 04:40 |
| Official URL: | http://dx.doi.org/10.1007/s00299-008-0525-x |
| URI: | http://eprints.icrisat.ac.in/id/eprint/10263 |
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