Bcl-xL transformed peanut (Arachis hypogaea L.) exhibits paraquat tolerance

Chu, Y. and Deng, X.Y. and Faustinelli, P. and Ozias-Akins, P. (2008) Bcl-xL transformed peanut (Arachis hypogaea L.) exhibits paraquat tolerance. Plant Cell Reports, 27 (1). pp. 85-92.

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Abstract

The human Bcl-xL gene was transformed into peanut cultivar Georgia Green via microprojectile bombardment. Following selection on hygromycin-containing medium and regeneration, eighty hygromycin-resistant callus clusters were recovered. Southern blot analysis of ten fertile lines revealed multiple insertions of the Bcl-xL transgene in most lines. Western blot analysis of primary plants and T1 progenies demonstrated detectable levels of Bcl-xL expression in four transgenic lines. We could not detect Bcl-xL protein in other tested lines even though transcripts were identified by RT-PCR and northern blot. Three of the western-positive transgenic lines either were sterile or the progenies lost the expressive copy of Bcl-xL. Only T1 progenies from line BX25-4-2a-19 continued to express an intermediate level of Bcl-xL. This line demonstrated paraquat tolerance at the 5 µM level. Tolerance to salt of T1 and T2 seeds from seven other transgenic lines also was tested, but no tolerance was found in these lines. A high level of Bcl-xL transgene expression may be deleterious to plant growth and development even though the gene may confer tolerance to other abiotic and biotic stresses such as drought and pathogens

Item Type:	Article
Additional Information:	SNNigam Collection
Author Affiliation:	Department of Horticulture, The University of Georgia, Tifton, GA 31973-0748, USA
Subjects:	Plant Protection Crop Improvement
Divisions:	Groundnut
Depositing User:	Mr B Krishnamurthy
Date Deposited:	17 Apr 2013 12:25
Last Modified:	17 Apr 2013 12:25
Official URL:	http://dx.doi.org/10.1007/s00299-007-0444-2
URI:	http://eprints.icrisat.ac.in/id/eprint/10260

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