Magbanua, Z.V. and Wilde, H.D. and Roberts, J.K. and Chowdhury, K. and et al, . (2000) Field resistance to Tomato spotted wilt virus in transgenic peanut (Arachis hypogaea L.) expressing an antisense nucleocapsid gene sequence. Molecular Breeding, 6 (2). pp. 227-236.
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Abstract
Peanut (Arachis hypogaea) lines transgenic for the antisense nucleocapsid (N) gene of a tomato spotted wilt virus (TSWV) strain isolated from peanut were generated by microprojectile-mediated transformation of repetitive somatic embryos of cultivars VC1 and AT120. The selectable marker (hygromycin resistance) and the N gene were on separate plasmids. A total of 207 VC1 and 120 AT120 hygromycin-resistant lines were produced. Of all the VC1 plants recovered 71% were cotransformed with the N gene (N+), but all plants were sterile. For AT120, 48 of the transgenic cell lines converted into plants. Polymerase chain reaction (PCR) screening showed 15 of the lines were transgenic for the N gene (N+), and two of these lines were fertile. A field test was conducted in 1998 at Ashburn, Georgia, using seeds from each fertile line, along with segregated and non-transgenic controls. Plants from four randomly selected field plots were examined for symptoms and analysed by double-antibody sandwich enzyme-linked immunoabsorbent assay and PCR at 10 and 14 weeks after planting. At 14 weeks, 76% of the N+ plants were symptomless, while 2% were severely symptomatic or dead. In contrast, only 42% of the plants lacking the N gene were symptomless and 50% were severely symptomatic or dead. Northern blot analysis of selected field-resistant plants detected transgene RNA, and the transcript level appeared undiminished after viral exposure
| Item Type: | Article |
|---|---|
| Additional Information: | SNNigam Collection |
| Uncontrolled Keywords: | antisense DNA, co-transformation, nucleocapsid gene, pathogen-derived resistance, somatic embryogenesis, transformation |
| Author Affiliation: | Department of Crop and Soil Sciences, University of Georgia, Athens, GA 30602, USA. |
| Subjects: | Plant Protection Crop Improvement |
| Divisions: | Groundnut |
| Depositing User: | Mr B Krishnamurthy |
| Date Deposited: | 17 Apr 2013 12:20 |
| Last Modified: | 17 Apr 2013 12:20 |
| Official URL: | http://dx.doi.org/10.1023/A:1009649408157 |
| URI: | http://eprints.icrisat.ac.in/id/eprint/10259 |
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