Introgression analysis of an interspecific hybrid population in peanuts (Arachis hypogaea L.) using RFLP and RAPD markers

Garcia, G.M. and Stalker, H.T. and Kochert, G. (1995) Introgression analysis of an interspecific hybrid population in peanuts (Arachis hypogaea L.) using RFLP and RAPD markers. Genome, 38 (1). pp. 166-176.

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Abstract

Forty-six introgression lines (F10C9) from a cross between Arachis hypogaea (2n = 4x = 40) and A. cardenasii (2n = 2x = 20) were analysed for the introgression of A. cardenasii chromosome segments. Seventy-three RFLP probes and 70 RAPD primers, expressing from one to four A. cardenasii-specific bands, were used to evaluate the set of introgression lines. Thirty-four RFLP probes and 45 RAPD primers identified putative A. cardenasii introgressed chromosome segments in one or more lines. Introgressed segments were detected by RFLP analysis in 10 of the 11 linkage groups; the smallest introgressed fragments were detected by single RFLP markers and the largest were detected by three of four adjacent markers and represented introgressed segments of 30-40 cM. Similar results were obtained with RAPD markers, although markers detecting introgressed fragments could not be placed on the peanut linkage map. Introgression into both A. hypogaea genomes was detected and its implication in breeding for disease resistance is discussed.

Item Type: Article
Additional Information: SNNigam Collection
Uncontrolled Keywords: Peanut, Arachis hypogaea, Arachis cardenasii, RFLPs, RAPDs, Introgression, Reciprocal recombination, Translocation, Alien gene transfer, Wide cross
Author Affiliation: Department of Crop Science, North Carolina State University, Raleigh, USA.
Subjects: Crop Improvement
Divisions: Groundnut
Depositing User: Mr Arbind Seth
Date Deposited: 17 Apr 2013 08:47
Last Modified: 17 Apr 2013 08:47
Official URL: http://dx.doi.org/10.1139/g95-021
URI: http://eprints.icrisat.ac.in/id/eprint/10216

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