Voytas, D.F. (2013) Plant Genome Engineering with Sequence-Specific Nucleases. Annual Review of Plant Biology, 64 (30). pp. 1-24.
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Abstract
Recent advances in genome engineering provide newfound control over a plant’s genetic material. It is now possible for most bench scientists to alter DNA in living plant cells in a variety of ways, including introducing specific nucleotide substitutions in a gene that change a protein’s amino acid sequence, deleting genes or chromosomal segments, and inserting foreign DNA at precise genomic locations. Such targeted DNA sequence modifications are enabled by sequence-specific nucleases that create double-strand breaks in the genomic loci to be altered. The repair of the breaks, through either homologous recombination or nonhomologous end joining, can be controlled to achieve the desired sequence modification. Genome engineering promises to advance basic plant research by linking DNA sequences to biological function. Further, genome engineering will enable plants’ biosynthetic capacity to be harnessed to produce the many agricultural products required by an expanding world population.
| Item Type: | Article |
|---|---|
| Additional Information: | D.F.V. would like to thank the members of the Voytas lab, past and present, for their many contributions to the field of plant genome engineering. Funding for ongoing research in the Voytas lab is provided by the National Science Foundation (DBI 0923827) and the National Institutes of Health (R01 GM098861). |
| Uncontrolled Keywords: | zinc-finger nucleases, transcription activator-like effector nucleases, TALENs, meganucleases, nonhomologous end joining, homologous recombination, gene targeting |
| Author Affiliation: | Department of Genetics, Cell Biology, and Development and Center for Genome Engineering, University of Minnesota, Minneapolis, Minnesota 55455; |
| Subjects: | Crop Improvement |
| Divisions: | General |
| Depositing User: | Mr Balakrishna Garadasu |
| Date Deposited: | 03 Apr 2013 13:43 |
| Last Modified: | 03 Apr 2013 13:43 |
| Official URL: | http://dx.doi.org/10.1146/annurev-arplant-042811-1... |
| URI: | http://eprints.icrisat.ac.in/id/eprint/10138 |
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