Factors affecting mechanical transmission of tomato spotted wilt virus to peanut (Arachis hypogaea)

Mandal, B. and Pappu, H. R. and Culbreath, A. K. (2001) Factors affecting mechanical transmission of tomato spotted wilt virus to peanut (Arachis hypogaea). Plant Disease , 85 (12). pp. 1259-1263.

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Abstract

Evaluation of groundnut germplasm for tomato spotted wilt virus (TSWV) resistance has been slowed by the difficulty in achieving a high rate of mechanical transmission of the virus to groundnut. In this study, improvements were made and a highly efficient mechanical transmission protocol was developed. Several factors that affect the transmission efficiency were identified. Use of two antioxidants (sodium sulfite and mercaptoethanol) and two abrasives (Celite and Carborundum) and application of the inoculum by rubbing with a cotton swab dipped in the inoculum as well as pricking with an inoculation needle resulted in a significantly higher transmission rate. The most susceptible growth stage of groundnut to TSWV inoculation was 2 to 3 days after germination (6 to 7 days after planting). The inoculation protocol consistently resulted in a higher percentage of infected plants from different sources of inoculum such as infected groundnut, tobacco and tomato.

Item Type:	Article
Additional Information:	Dr S N Nigam research collection - Box No:17
Uncontrolled Keywords:	Bunyaviridae, Georgia Runner, Groundnut, Host plant resistance, Tospovirus
Author Affiliation:	Department of Plant Pathology, University of Georgia, Coastal Plain Experiment Station, USA.
Subjects:	Crop Improvement
Divisions:	Groundnut
Depositing User:	Mr Arbind Seth
Date Deposited:	29 Mar 2013 05:57
Last Modified:	29 Mar 2013 05:57
Official URL:	http://dx.doi.org/10.1094/PDIS.2001.85.12.1259
URI:	http://eprints.icrisat.ac.in/id/eprint/10043

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